Human IgE (non-immune)
Human monoclonal antibody (non-immune)
Cat.No. DIA HE1
Preparation: Protein L purified
Content: 100 µl, 1mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.15 M NaCl
Storage: For long term storage, -18 to -22ºC is recommended, but it may be stored for short term use at 2 to 8ºC. The expiry date on the vial refers to storage at -18 to -22ºC. Repeated freeze/thawing is not recommended
This product is purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B.
Must be handled as potentially infectious as all human material.
The purity of the human IgE is > 98%, tested by SDS-PAGE.
As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.
Non-immune
This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.
Larger Picture (113kB)
Picture: Lane 1 shows molecular weight markers, lane 2 Diatec purified human IgE, batch 307, MW 185 000, lane 3 shows myeloma source IgE, purified from human serum.
Downloads
DIA HE1.pdf (32.96 kB) Product specification
MSDS DIA HE1.pdf (54.35 kB)
Flyer IgE.pdf (302.37 kB)
IgE flyer
